"11 plantes médicinales de La Réunion révèlent des effets anti-inflammatoires et régulateurs du métabolisme chez le poisson-zèbre, avec Hypericum lanceolatum et Ayapana triplinervis comme espèces prometteuses contre le stress oxydatif et l’accumulation de lipides."
Cette étude menée par l'équipe de Nicolas Diotel (MCU) explore le potentiel thérapeutique de 11 plantes médicinales de La Réunion, traditionnellement utilisées pour soigner les blessures, l’inflammation et les maladies métaboliques. Les extraits aqueux de ces plantes, riches en polyphénols (acide chlorogénique, gallique, quercétine, etc.), ont été testés in vivo sur des modèles de poisson-zèbre pour évaluer leur toxicité et leurs effets biologiques. Les résultats révèlent des propriétés anti-inflammatoires, régénératrices et anti-lipidiques à des concentrations non toxiques, avec des performances notables pour Hypericum lanceolatum et Ayapana triplinervis. Ces travaux ouvrent des perspectives pour le développement de stratégies naturelles contre les maladies chroniques et métaboliques.
Mots-clés : Plantes médicinales, Polyphénols, Régénération, Inflammation, Accumulation lipidique.
Mots-clés : Plantes médicinales, Polyphénols, Régénération, Inflammation, Accumulation lipidique.
Biomed Pharmacother
2025 Aug:189:118305.
doi: 10.1016/j.biopha.2025.118305. Epub 2025 Jul 8.
Toxicity and therapeutic effects of aqueous extracts
from reunionese medicinal plants: Insights from zebrafish models
Laura Gence 1, Danielle Fernezelian 1, Tatiana Al Anaissy 1, Marie Laurine Apalama 1, Anaïs Hoarau 1, Batoul Ghaddar 1, Matthieu Bringart 1, Bryan Veeren 1, Olivier Meilhac 2, Jean-Loup Bascands 3, Nicolas Diotel 4
Affiliations
ABSTRACT
Background: Plant-derived compounds have long been used to treat wounds, inflammation and metabolic diseases, due to their broad biological activities making them valuable candidates for new therapies. Réunion Island has numerous medicinal plants listed in the French pharmacopoeia but limited information exists on their chemical composition and biological effects.
Aims: This study focused on 11 traditional medicinal plants from Réunion Island (Antirhea borbonica, Aphloia theiformis, Ayapana triplinervis, Coffea mauritiana, Dodonaea viscosa, Hypericum lanceolatum, Nuxia verticillata, Olea europaea, Psiloxylon mauritianum, Syzygium cumini, and Vepris lanceolata). We aimed to evaluate the in vivo toxicity of the aqueous extracts of these medicinal plants and assess their biological effects using zebrafish models.
Methods and results: Polyphenol concentrations and compositions were determined using the Folin-Ciocalteu colorimetric assay and liquid chromatography-mass spectrometry. The total polyphenol content ranged from 103.2 to 14.2 mg GAE/g dried plant and the main identified compounds included chlorogenic acid, gallic acid, quercetin, kaempferol, and mangiferin. Toxicity assays allowed the determination of the maximum non-toxic concentration (MNTC) for each extract. At these concentrations, several extracts showed beneficial effects on inflammation, tissue repair, and lipid accumulation.
Conclusion: This work presents the first comprehensive analysis of the composition and in vivo activity of these 11 Reunionese medicinal plants. It demonstrates the therapeutic value of such extracts in managing oxidative stress, enhancing regeneration, modulating immune responses, and reducing lipid accumulation in vivo. Hypericum lanceolatum and Ayapana triplinervis appeared as particularly promising. These findings support further exploration of plant-based strategies for treating chronic and metabolic diseases and their complications in a preventive/therapeutic approach.
Keywords: Lipid accumulation; Polyphenols; Regeneration; Tail-injury; Toxicity.
Affiliations
- 1Université de La Réunion, INSERM, UMR 1188, Diabète athérothrombose Thérapies Réunion Océan Indien (DéTROI), Saint-Pierre, La Réunion, France.
- 2Université de La Réunion, INSERM, UMR 1188, Diabète athérothrombose Thérapies Réunion Océan Indien (DéTROI), Saint-Pierre, La Réunion, France; CHU de La Réunion, Saint-Denis, La Réunion, France.
- 3Université de La Réunion, INSERM, UMR 1188, Diabète athérothrombose Thérapies Réunion Océan Indien (DéTROI), Saint-Pierre, La Réunion, France. Electronic address: [email protected].
- 4Université de La Réunion, INSERM, UMR 1188, Diabète athérothrombose Thérapies Réunion Océan Indien (DéTROI), Saint-Pierre, La Réunion, France. Electronic address: [email protected].
ABSTRACT
Background: Plant-derived compounds have long been used to treat wounds, inflammation and metabolic diseases, due to their broad biological activities making them valuable candidates for new therapies. Réunion Island has numerous medicinal plants listed in the French pharmacopoeia but limited information exists on their chemical composition and biological effects.
Aims: This study focused on 11 traditional medicinal plants from Réunion Island (Antirhea borbonica, Aphloia theiformis, Ayapana triplinervis, Coffea mauritiana, Dodonaea viscosa, Hypericum lanceolatum, Nuxia verticillata, Olea europaea, Psiloxylon mauritianum, Syzygium cumini, and Vepris lanceolata). We aimed to evaluate the in vivo toxicity of the aqueous extracts of these medicinal plants and assess their biological effects using zebrafish models.
Methods and results: Polyphenol concentrations and compositions were determined using the Folin-Ciocalteu colorimetric assay and liquid chromatography-mass spectrometry. The total polyphenol content ranged from 103.2 to 14.2 mg GAE/g dried plant and the main identified compounds included chlorogenic acid, gallic acid, quercetin, kaempferol, and mangiferin. Toxicity assays allowed the determination of the maximum non-toxic concentration (MNTC) for each extract. At these concentrations, several extracts showed beneficial effects on inflammation, tissue repair, and lipid accumulation.
Conclusion: This work presents the first comprehensive analysis of the composition and in vivo activity of these 11 Reunionese medicinal plants. It demonstrates the therapeutic value of such extracts in managing oxidative stress, enhancing regeneration, modulating immune responses, and reducing lipid accumulation in vivo. Hypericum lanceolatum and Ayapana triplinervis appeared as particularly promising. These findings support further exploration of plant-based strategies for treating chronic and metabolic diseases and their complications in a preventive/therapeutic approach.
Keywords: Lipid accumulation; Polyphenols; Regeneration; Tail-injury; Toxicity.